Multispectral Optoacoustic Tomography (MSOT) utilizes the photoacoustic effect to visualize and quantify anatomical, functional and molecular information, in vivo, in deep tissue and in real time. Today, MSOT allows the study of disease processes on a molecular level as well as the analysis of pharmacokinetic and biodistribution properties for new substances in small animals. For the future, MSOT also promises to become a valuable tool for clinical diagnostics. This webinar provides an overview on MSOT technology as well as applications in preclinical research and an outlook towards clinical translation.