Optoacoustic imaging (OAI) is an innovative technique that facilitates the acquisition and analysis of structural, functional and molecular information in small animals. In OAI an animal is illuminated with pulsed laser light. Absorbance of light energy by tissue results in the emission of acoustic signals via the so-called ‘photoacoustic effect’ that describes the conversion of light energy into sound waves. Acoustic signals detected are then reconstructed to form a cross-sectional or volumetric image of light absorbance by the tissue of interest. This webinar focuses on the range of translational and multi-scale applications where biomedical research can benefit from OAI.