Lymphedema is a chronic and debilitating disorder affecting millions of people around the world. While typically managed well, there is a need for lymphatic surgery when conservative therapy fails. Optoacoustic imaging has the potential to be a useful tool to trace lymph vessels and differentiate them from the vascular network. In this webinar, Dr. Guido Giacalone, Head of the Department of Lymphatic Surgery at AZ Sint-Maarten Hospital (Mechelen, Belgium), outlines his work in the field and the use of MSOT for preoperative mapping of lymphatic and blood vessels.